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The ability of E7449 to inhibit the activity of human recombinant PARP1, mouse recombinant PARP2 or human recombinant TNKS1 was resolute making use of chemiluminescent PARP or tankyrase assay kits from Trevigen, pursuing the manufacturer's Directions.In contrast, Parp1 knockout mice clearly show worse survival with a higher-Unwanted fat diet regime